Use the following worksheet to help keep track of the searches conducted, experts consulted, and justifications if an alternative is found, but why it will not work for your research, teaching, or breeding protocol.
The use of this worksheet is completely voluntary; it is not submitted to the IACUC for a proposal review.
However, it can be used to assist with determining resources to consult and develop effective search strategies to compile 3Rs related information for the IACUC protocols at Virginia Tech.
Check out UNC's Health Sciences Library guide on "Searching the Literature for Animal Testing Alternatives" (UNC Health Sciences Library) - provides a step-by-step literature review process specifically for the 3Rs/alternatives.
As stated in the USDA Animal Care Policy Manual (Policy 12):
To comply with the mandates, the alternatives literature search sections for each IACUC protocol at Virginia Tech must include:
As noted, other sources besides databases may be used to locate alternatives, including:
Record any non-database sources in the IACUC worksheet (download through the link in the upper left hand corner of this page) and make sure to note in the protocol any additional sources outside of the database searches that have been consulted.
REDUCE: minimize how many animals are used.
REFINE: use techniques/procedures that minimize distress and pain to animals.
REPLACE: substitute animal models with non-animal techniques or lower organisms.
Adapted from: Russel, WMS and RL Burch (1959). The Principles of Humane Experimental Technique. Universities Federation of Animal Welfare: England, 238p.